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Regeneration capacity of 35 tetraploid alfalfa (Medicago sativa L.) cultivars representing all of the nine germplasm sources of U.S. alfalfa is reported. Approximately 20 genotypes from each cultivar were tested for regeneration using hypocotyl derived callus on a standard media sequence. Ladak, Norseman, Turkistan, and Nomad were clearly superior sources of regenerating genotypes; however, a wide spectrum of varieties possessed genotypes that would regenerate. This information should predict sources of potential regenerators among modern alfalfa cultivars.
Key Words: Medicago sativa L. Differentiation Genetic variation Callus
Received for publication August 22, 1983.
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