Crop Science Journal of Natural Resources and Life Sciences Education
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Published in Crop Sci 24:943-945 (1984)
© 1984 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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In Vitro Regenerative Potential of Alfalfa Germplasm Sources1

D. H. Mitten, S. J. Sato and T. A. Skokut2

Regeneration capacity of 35 tetraploid alfalfa (Medicago sativa L.) cultivars representing all of the nine germplasm sources of U.S. alfalfa is reported. Approximately 20 genotypes from each cultivar were tested for regeneration using hypocotyl derived callus on a standard media sequence. ‘Ladak’, ‘Norseman’, ‘Turkistan’, and ‘Nomad’ were clearly superior sources of regenerating genotypes; however, a wide spectrum of varieties possessed genotypes that would regenerate. This information should predict sources of potential regenerators among modern alfalfa cultivars.

Key Words: Medicago sativa L. • Differentiation • Genetic variation • Callus


1 Contribution from the Monsanto Agric. Products Co., 800 N. Lindbergh Blvd., St. Louis, MO 63167.

2 Research scientists.

Received for publication August 22, 1983.





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Copyright © 1984 by the Crop Science Society of America.