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Genetic Shifts in Mixed Bean Populations. II. Effects of Regeneration¹

Seed storage is the most commonly accepted form of long-term germplasm preservation. Maintenance of the genetic integrity of seed populations during both seed storage and regeneration is an important aspect of this preservation process. Seeds of mixed and pure-line populations of eight snap bean (Phaseolus vulgaris L.) cultivars were artificially aged and grown in the field to determine potential genetic shifts in the populations. After three cycles of seed regeneration the composition of unaged populations gradually shifted in favor of three cultivars (‘Black Valentine’, ‘Tendercrop’, and ‘Cherokee Wax’); while artificially aging seeds for 6 weeks at 32°C, 90% relative humidity, resulted in the total elimination of three cultivars (‘Kinghorn Wax’, Tendercrop, and ‘White Seeded Tendercrop’). Averaged over 3 years, Black Valentine had the highest seed yield/plant followed by Tendercrop and Cherokee Wax with ‘Bountiful’ having the lowest yield. Computer simulation of the effects of repeated cycles of regeneration of unaged seed populations predicted that Bountiful would be eliminated from the population after 5 cycles, assuming a sample size of 64 seeds for each cycle. Kinghorn Wax, ‘Spartan Arrow’ and ‘Landreth's Stringless Green Pod’ would be eliminated after 9, 10, and 15 cycles, respectively. Simulation of the combined effects of seed aging (to 50% viability) and regeneration resulted in a prediction that only two cultivars (Black Valentine and Cherokee Wax) would survive 15 cycles.

Key Words: Seed germination • Seed yield • Seed deterioration • Germplasm preservation • Computer simulation • Phaseolus vulgaris

² Plant physiologist, USDA-ARS, National Seed Storage Lab., Fort Collins, CO 80523.

Received for publication May 13, 1983.