Crop Science Journal of Natural Resources and Life Sciences Education
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Published in Crop Sci 22:970-972 (1982)
© 1982 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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The Effect of Rhizobium trifolii Strains and Crimson Clover Genotypes on N2 Fixation1

G. R. Smith, W. E. Knight, H. L. Peterson and C. Hagedorn2

The effects of crimson clover (Trifolium incarnatum L.) genotype and strain of Rhizobium trifolii on dry weight yield and N2 fixation were investigated in a factorial experiment. The treatments were arranged in a randomized complete block design with eight replications. The clover-Rhizobium combinations were grown in plastic pouches with a N-free nutrient solution. A growth chamber was used to maintain 24/19 C diurnal temperatures and a 12 hour photoperiod.

After 60 days of growth, the combinations were assayed for N2 fixation using acetylene reduction and the plant tops were harvested and dried. Plant lines, strains of Rhizobium and line x strain interaction were all significant sources of variation for both acetylene reduction rate and dry weight yield. Strains had the largest effect, in terms of variance components, on dry weight yield followed by plant lines and strain x line interaction, respectively. Strains also had the largest effect on acetylene reduction rate followed by strain x line interaction and plant lines, respectively. Strain TA1 of R. trifolii was highly variable in its effect on the different plant lines. Response of different inbred lines with this strain ranged from efficient to inefficient.

Key Words: Acetylene reduction • Trifolium incarnatum L. • N2 fixation efficiency


1 Cooperative investigations of the Mississippi Agric. and Forestry Exp. Stn. and ARS-USDA, Mississippi State, MS 39762.

2 Formerly agronomist (now assistant professor, Texas A&M Univ. P.O. Drawer E, Overton, Tx 75684) and research agronomist, Crop Science and Engineering Res. Lab., ARS-USDA and associate agronomists, Mississippi Agric. and Forestry Exp. Stn., Mississippi State, Mississippi 39762.

Received for publication September 8, 1981.





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Copyright © 1982 by the Crop Science Society of America.