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Published in Crop Sci 22:333-337 (1982)
© 1982 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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Enzyme-Linked Immunosorbent Assay for the Determination of Lipoxygenase Isoenzymes in Soybean1

Seizo Yabuuchi, Richard M. Lister, Bernard Axelrod2, James R. Wilcox and Niels C. Nielsen3

An enzyme-linked immunosorbent assay (ELISA) was developed for the quantitative determination of lipoxygenase-1 (L1) and lipoxygenase- 3 (L3) in soybeans (Glycine max (L.) Merr.). Monospecific goat (Carpa hircus) and rabbit (Oryctolagus cuniculus) immunoglobulins purified by affinity chromatography were used for the L1 and L3 assays, respectively. In both cases the coefficients of variation in the assays were about 10%. A linear relationship between lipoxygenase (L1) enzymic activity and the enzyme protein content as determined by ELISA was obtained. The technique has considerable potential for screening of segregating soybean populations for genotypes deficient in specific lipoxygenase isozymes.

Key Words: ELISA • immunological screening • Glycine max (L.) Merr. • oil quality

1 Contribution of the USDA and the Purdue Agric. Exp. Stn. Published as Paper No. 3546, Journal Series, Purdue Agric. Exp. Stn. Mention of a proprietary product does not constitute its guarantee or warranty by either Purdue Univ. or USDA-SEA. Financial support from the American Soybean Association Research Foundation and NSF (Grant PCM 80-11820) are gratefully acknowledged.

2 Respectively, research associate, Agronomy Dep. (presently, Central Research Laboratories, Asahi Breweries, Ltd., Tokyo, Japan), professor of botany and plant pathology, and professor of biochemistry, Purdue Univ., W. Lafayette, IN 47907.

3 Respectively, research geneticist, AR, SEA, USDA, and professor and assistant professor of agronomy, Purdue Univ., W. Lafayette, IN 47907.

Received for publication May 14, 1981.




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