HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Lesar, L. E. Articles by Peterson, D. M. Search for Related Content PubMed Articles by Lesar, L. E. Articles by Peterson, D. M. Agricola Articles by Lesar, L. E. Articles by Peterson, D. M.

Growth and Composition of Kernels Developing on Excised Oat Panicles in Liquid Culture¹

Excised oat (Avena sativa L., cv. ‘Froker’) panicles were cultured in defined media for 12 days, beginning 8 days after heading, to determine effects of supplied culture nutrients on growth, development, and composition of the grain. The sterile media contained 55.6 mM sucrose, mineral salts, vitamins, and various sources and levels of N. Dry weight increases of the groats were near normal when the N source was mixed amino acids, glutamine, or NH₄NO₃. The N concentration of the groats was increased to 3.85, 4.30, and 5.08% with mixed amino acids, glutamine, and NH₄ NO₃, respectively, after 12 days in culture, as compared to a N concentration of about 2.96% for groats on intact plants. Electrophoresis and amino acid analysis of total groat protein revealed no marked differences between cultured and intact plants. The percentage of N in the bran fraction was increased by mixed amino acids medium, and that of the.endosperm by glutamine medium. There was no increase in nitrate in groats of the cultured panicles. Levels of soluble -amino N were increased slightly by the glutamine medium. It was concluded that the panicle culture technique is valid for study of the effects of supplied nutrients on grain composition. Groat N is responsive to the level of media N, and glutamine or NH₄NO₃ adequate as a N source.

Key Words: Avena sativa L. • Amino acids • Glutamine • Ammonium nitrate • Storage protein • Seed Development

² Graduate research assistant, Dep. of Agronomy, Univ. of Wisconsin, Madison, WI 53706; plant physiologist, USDA, SEA-AR and assoc. professor of agronomy, Univ. of Wisconsin, Madison, WI 53706.

Received for publication January 13, 1981.