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Published in Crop Sci 21:347-348 (1981)
© 1981 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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Effects of Different Types of Sand and Vermiculite Grades on the Test Tube Method for Evaluating Preinoculated Seed1

J. F. Schoen and N. Kramer2

Combinations of two types of sand and two grades of vermiculite in test tubes (25 x 200 mm) were evaluated for growth of seedlings with root nodules at 21 days of age. Rhizobium-inoculated alfalfa seeds were planted two per test tube. Each type of sand was suitable for testing provided it was washed free of fine particles. Seedlings grown in substrate combinations which included the vermiculite having smaller (0.32 cm) particle sizes produced fewer nodules regardless of the sand type.

Key Words: Medicago sativa L. • Growing medium • Emergence • Nodulation

1 Cooperative investigation. Seed Standardization Branch, LPG&S Division, AMS, USDA, and Bureau of Laboratory Services, Dep. of Agriculture, Trade & Consumer Protection, Madison, WI.

2 Plant pathologist, USDA, and plant disease specialist, Dep. of Agriculture, respectively.

Received for publication July 23, 1980.




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