Crop Science Journal of Natural Resources and Life Sciences Education
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Published in Crop Sci 16:731-732 (1976)
© 1976 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Burton, G. W.
Right arrow Articles by Hanna, W. W.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Burton, G. W.
Right arrow Articles by Hanna, W. W.
Agricola
Right arrow Articles by Burton, G. W.
Right arrow Articles by Hanna, W. W.

Ethidium Bromide Induced Cytoplasmic Male Sterility in Pearl Millet1

Glenn W. Burton and Wayne W. Hanna2

Seeds of homozygous ‘Tift 23DB1’ pearl millet [Pennisetum americanum (L.) K. Schum], maintainer for cytoplasmic male sterile (cms) ‘Tift 23DA1,’ were soaked in deionized water solutions of 250 and 1,000 ppm of ethidium bromide (EB) at 5 C for 40 hours. Treated seeds were rinsed in tap water for 30 min, surface-dried, and planted in replicated two-row plots in the field with two-row plots of untreated Tift 23DB1. Male-sterile mutants (whole heads, sectored heads, or heads with intermingled male-sterile and male-fertile florets) occurred at frequencies of 1 in 30,100, I in 515, and 1 in 274 in total head populations of 30,100, 22,700, and 19,200 treated with 0, 250, and 1,000 ppm EB, respectively. Occurrence of M1 sterile mutants that set seed when pollinated and gave rise to some cms progenies in the greenhouse proved that the mutants were cytoplasmic male sterile.

Key Words: Tift 23DB1 • Tift 23DA1 • Hybrid vigor • Pennisetum americanum

1 Cooperative investigations of the ARS, USDA, and the Univ. of Georgia, College of Agric. Exp. Stns., Coastal Plain Station, Tifton, Ga. Study was supported in part by U.S. Energy Research and Development Administration Contract No. AT (38-1)-637; FAO/IAEA, Vienna, Austria. The authors gratefully acknowledge the suggestions of C. Broertjes, Inst. for Atomic Sciences in Agric., Wageningen, Netherlands; and of A. Ashri, The Hebrew Univ., Rehovot, Israel, relative to the choice of a mutagenic agent for this research.

2 Research geneticists, ARS, USDA, and the Univ. of Georgia, College of Agric. Exp. Stn., Coastal Plain Station, Tifton, Ga.

Received for publication April 13, 1975.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
The SCI Journals Agronomy Journal Vadose Zone Journal
Journal of Natural Resources
and Life Sciences Education		 Soil Science Society of America Journal
Journal of Plant Registrations Journal of
Environmental Quality		 The Plant Genome
Copyright © 1976 by the Crop Science Society of America.